| Steps in Classical Biological Control |
Completed Activities |
Future Activities |
| 1.) Identify the pest |
1.) Metamasius callizona |
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| 2.) Identify the origin of the pest |
2.) Mexico and Central America |
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| 3.) Survey for natural enemies in place of origin of the pest |
3.) Surveys conducted in Mexico, Panama, and Guatemala |
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| 4.) Identify potential biological control agent(s) |
4.) Lixophaga sp. (tachinid fly) |
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| 5.) Import to quarantine facility with required permits |
5.) Lixophaga sp. was imported to DPI quarantine in Gainesville in late 1999 and late 2000 |
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| 6.) Establish colony in quarantine |
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6.) Need to establish colony of Lixophaga sp. in quarantine |
| 7.) Non-target testing in quarantine |
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7.) Begin testing with M. hemipterus and M. mosieri (laboratory colonies of both have been established in preparation for these tests) |
| 8.) Apply for release permit |
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8.) If weevil does not attack other Metamasius species, application will be made for a release permit |
| 9.) Rear biological control agent in large numbers |
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9.) Rear fly in laboratory and/or greenhouses outside of quarantine |
| 10.) Release in areas of high pest population density |
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10.) Determine best release sites (selected state parks) and release fly |
| 11.) Evaluate effects of biological control agent |
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11.) Put out weevil-infested plants for later determination of percent parasitism; measure population densities of weevil before and after release |
| 12.) Modify release plan and make further releases |
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12.) Adjust releases sites, schedule, methods, etc. |